Arts

• John Mitchell, American jazz banjoist who worked with Johnny Dunn
• John Mitchell, Canadian ice dancer in the 1962 World Figure Skating Championships
• John Mitchell (minister) (1794–1870), American minister and author
• John Mitchell (musician) (born 1973), lead singer and guitarist for UK band It Bites, and music producer
• John Ames Mitchell (1844–1918), American illustrator
• John Cameron Mitchell (born 1963), American filmmaker
• John Campbell Mitchell (1861–1922), Scottish landscape artist
• John Grant Mitchell (actor) (1874–1957), American character actor in the 1930s and 1940s
• John Hanson Mitchell (born 1940), American author
• John Mitch Mitchell (1946–2008), English musician
• John R. Mitchell (poet) (1940–2006), poet and a professor of English
• John W. Mitchell (1917–2005), British sound engineer
• John Wendell Mitchell (1880–1951), Canadian author

Business

• John Mitchell Jr. (1863–1929), editor of the Richmond Planet, a newspaper located in the Jackson Ward community of Richmond, Virginia
• John Mitchell (United Mine Workers) (1870–1919), president of the United Mine Workers
• John Francis Mitchell (1928–2009), vice chairman of Motorola and inventor of the mobile cell phone
• John G. Mitchell (editor) (died 2007), American editor of National Geographic magazine
• John J. Mitchell (banker) (1853–1927), a banker based in Chicago and president of the Illinois Trust and Savings Bank
• Jack Mitchell (banker) (1897–1985), American banker and co-founder of United Airlines
• John Mitchell (merchant) (1786–1881), Glasgow cork merchant and ship owner

Military

• John Mitchell (born 1785) was a British Army officer and author.
• John Mitchell (Medal of Honor) served as an American soldier during the Indian Wars and received the Medal of Honor. He was part of Company I, 5th US Infantry.
• John Mitchell (RAF officer) (1888–1964) was an officer in the Royal Air Force.
• John G. Mitchell (general) (1838–1894) was a general in the American Civil War.
• John J. Mitchell (Medal of Honor) (1846–1898) was an American soldier during the Indian Wars and received the Medal of Honor. He was part of Company L, 8th US Cavalry.
• John K. Mitchell (1811–1889) was a commander in the Confederate Navy during the American Civil War. He is associated with the USS Alpha (1864).
• John Lewis Mitchell (1918–2016) was an RAF Air Commodore.
• John R. C. Mitchell was a United States naval aviator. He is associated with June 1955.
• John W. Mitchell (United States Air Force) (1915–1995) was a US Air Force officer, a flying ace, and led Operation Vengeance.
• John Wesley Mitchell (1891–1969) was an Australian soldier.
• John Merrill (Medal of Honor) (1846–1883) was an American soldier during the Indian Wars and received the Medal of Honor.

Politics

• John F. Mitchell (1862–1943) was a member of the Manitoba provincial legislature from 1907 to 1910. He also served as a city council member and acted as mayor of Winnipeg, Manitoba, in 1900.
• John William Mitchell (1872–1952) was mayor of Calgary, Alberta, from 1911 to 1913. He also served as a city council member in Calgary from 1905 to 1911 and again in 1915.
• John Henry Mitchell was a Canadian politician who served as a member of the Ontario provincial legislature from 1926 to 1929 as part of the Liberal-Progressive party.
• John Mitchell (Ontario politician) was the first president and main leader of the Ontario Co-operative Commonwealth Federation from 1934 to 1941. He also served as a city council member in Hamilton, Ontario.

• John Mitchell (MP for City of London) (died 1445) was a member of the medieval Parliament of England in multiple sessions between 1411 and 1435.
• John Mitchell (MP for Truro) was a member of the Parliament of England during the Tudor period, specifically in the Parliament of 1563–1567.
• John Mitchell (Hull politician) (c. 1781–1859) was a member of Parliament for Kingston-upon-Hull from 1818 to 1826.
• John Mitchel (1815–1875) was an Irish journalist and politician who served as a member of Parliament for County Tipperary in 1875.
• John Thomas Whitehead Mitchell (1828–1895) was a British co-operative activist who led the Co-operative Congress in 1879 and 1892.

• John Mitchell (Pennsylvania politician) (1781–1849) was a member of the United States Congress from Pennsylvania.
• John Mitchell (United Mine Workers) (1870–1919) was a labor leader.
• John Mitchell Jr. (born 1954), also known as "Larry," is an American politician who has served in the Delaware House of Representatives.
• John H. Mitchell (1835–1905) was a U.S. senator from Oregon.
• John H. Mitchell (Iowa politician) (1899–1992) was an Iowa state representative and attorney general.
• John I. Mitchell (1838–1907) was a U.S. senator from Pennsylvania.
• John Joseph Mitchell (1873–1925) was a U.S. representative from Massachusetts.
• John L. Mitchell (1842–1904) was a Wisconsin politician and the father of Gen. Billy Mitchell.
• John M. Mitchell (1858–1905) was a U.S. representative from New York.
• John N. Mitchell (1913–1988) was the U.S. Attorney General and involved in the Nixon Watergate scandal.
• John Purroy Mitchel (1879–1918) was the mayor of New York.
• John Ridley Mitchell (1877–1962) was a U.S. representative from Tennessee.

• John Mitchell (Australian politician) (1869–1943) was a member of the Victorian Parliament.
• John Walker Mitchell (1832–1914) was a New Zealand politician born in Scotland.

Science

• John Mitchell (geographer) (1711–1768) was a colonial American physician, botanist, and geographer
• John Kearsley Mitchell (1798–1858) was an American physician
• John Mitchell (physicist) (1913–2007) was a New Zealand-born physicist
• J. Murray Mitchell (1928–1990) was an American climatologist
• John F. B. Mitchell (born 1948) is a British climatologist and climate modeller
• John C. Mitchell is a computer scientist
• John Mitchell (chemist) is an American chemist and materials scientist

Sports

• John Mitchell (outfielder) (1937–2020), Negro league outfielder
• John Mitchell (pitcher) (born 1965), Major League Baseball pitcher, 1986–1990
• Johnny Mitchell (baseball) (1894–1965), baseball shortstop, 1921–1925

• John Mitchell (ice hockey, born 1895) (1895–1957), Canadian ice hockey player with the Regina Capitals and Duluth Hornets
• John Mitchell (ice hockey, born 1985), National Hockey League player with the Colorado Avalanche

• John Mitchell (administrator), president of the Melbourne Football Club and the Melbourne Cricket Club
• John Mitchell (American football) (born 1951), American football player and coach
• John Mitchell (Australian footballer) (1891–1962), Australian rules footballer
• John Mitchell (cricketer) (born 1947), New Zealand cricketer
• John Mitchell (footballer, born 1800s) (active from 1879 to 1891), footballer for Doncaster Rovers, Newton Heath, and Bolton Wanderers
• John Mitchell (footballer, born 1952), English footballer
• John Mitchell (hurler) (born 1946), Irish retired hurler and manager
• John Mitchell (rugby union) (born 1964), rugby player and coach
• John T. Mitchell (1854–1914), whist and bridge player
• Johnny Mitchell (born 1971), American football tight end

Others

• John Mitchell (historian) (1941–2021), a historian from New Zealand
• John Murray Mitchell (missionary) (1815–1904), a Scottish missionary and expert in Eastern cultures
• John Mitchell (Being Human), a character in the television series Being Human
• John Matthew Mitchell (1925–2019), Assistant Director General, British Council